Fast Sensitive and Accurate Analysis of the Most Common Synthetic Food Colorants in 65 Egyptian Commercial Products Using New HPLC–DAD and UPLC-ESI–MS/MS Methods

Abstract Overexposure to food colorants above the allowed daily intake (ADI) level can provoke hyperactivity and other disturbed behaviors especially in children. Two new methods were developed separate five synthetic colorants, which Tartrazine (E102), Sunset Yellow (E110), Allura Red (E129), Carmoisine (E122), Brilliant Blue (E133). They are labeled on a large variety of commercial products Egyptian market without mentioning their definite concentrations. Therefore, there was real need determine these with simple, accurate, fast methods. This is first study wide present market. The HPLC approach photodiode array detection quantify C18 column, mobile phase composed acetonitrile water containing 1% ammonium acetate (pH 6.8), separation carried out using gradient program. eluted efficiently separated within 9 min. Then, as complementary technique HPLC, UPLC-ESI–MS/MS for identification accurate mass measurement found high concentrations, obtained simultaneously negative mode, run time only 3 These validated according ICH recommendations they applied analyze 65 including jelly powder, puddings, ice cream powders, concentrated soft drink carbonated drinks, chewing gums, sugar confectionery.